HB – 100 Future of analysis

Zivak Technologies has developed state of the art Hemoglobin A1c and variant Analyzer. This eliminates the errors from manual preparation steps. With HB – 100 Hemoglobin A1c and Variant Analyzer, the low throughput in chromatography analysis will not be a problem for the laboratories any more. Zivak HB – 100 system can inject from closed cap whole blood collection tubes. This eliminates the contamination risk from the opening tubes for the technician.

All sample preparation steps for analysis made by the unique modules of B – 100 Analyzer. The processes listed below can be done by robotic arms with these unique modules;

  • Rational Barcode reading from whole blood tube
  • Samples inversion for homogenization
  • Sample transfer from whole blood tube
  • Dilution and Hemolysis
  • Injection and data acqusition
  • System is ot required any human intervention during all steps.

Zivak HB – 100 system inverts the whole blood sample tubes for homogenisation before the sample preparation needed. The system automatically generates sample sequence by reading barcode from primary sample collection tube which barcode by hospital LIS system. Due to rotational barcode reading which followed by automated sample preparation, the system is independent from placing order to position of barcode of the samples in samples tray. PLACE YOUR SAMPLES AS YOU GET without align the bardcode. There is no possible sample mismatch from the transfer of the samples from primary samples tube to standard chromatography vials or well plates

HB – 100 Software Properties

  • User friendly graphical interface for easy interaction
  • Fast, automated data processing and automatic peak detection
  • Allow user to create, edit and print custom designed report
  • Automatic LIS Transfer of processed results

Technical Specifications

⇨ Power Requirments:

  • Operating Voltage: 220-230V AC
  • Operating Frequency: 50 – 60 Hz
  • Operating Current: 5,95A Max

⇨ Dimensions (W x D x H): 766mm x 714mm x 757mm
⇨ Working Temperature: 10 – 40 ℃
⇨ Storage Temperature: -20 70 ℃
⇨ Humidity %30 – 80RH
⇨ Sound Level: < 60dB @1m

Sampling & Injection

⇨ Sample Capacity: 110 (2ml VacuTube) samples with continuous loading
⇨ Loop Volume: 20uL
⇨ Analysis time: HBA1c 1,5 Min. Variant 6,5 Min.
⇨ Syringe volume: Standard size 50ul, 10mL
⇨ Wash Solvent: External wash solvent bottle.
⇨ Wetted parts in flow path: Metal tubing stainless steel number, PTFE, teflon, glass
⇨ Switching Time of valves: Less then 1 second
⇨ Column Oven: 35 ℃ Constant
⇨ Valves: 1 x 6 Port Injection Valve
⇨ Barcode Reader & Shaker: Barcode reading with rotational chamber which allows to read barcode anywhere on the tube. This module also has a optional shaker module for shaking blood tubes.
⇨ Emergency Sample Tray: 1 chamber for emergency samples and 2 chambers for calibrator & control samples

Analytical Performance

⇨ Injection Mode: Full – Loop
⇨ Injection Reproducibility: Full loop Injection < %0.3 RSD
⇨ Carry Over:

    • Less than %0.05 while standard wash
    • Less than %0.01 while extra wash


⇨ User Interface: Automass Chromatography Software V2
⇨ Injection Mode: Full – Loop
Injection volume: 20uL


      • Wash inside and outside of the middle
      • Wash between injections
      • Wash between vials


⇨ CIB to PC communication with CAT5 ethernet cable


⇨ 2 x HPLC Pumps
⇨ Flow Rates: 0.001 – 5.0 mL/min
⇨ Pressure: 6000 psi
⇨ Pressure Accuracy: ± 2% of full scale pressure
⇨ Pressure Zero Offset: ± 2psi
⇨ Flow Accuracy: ± 2 for a floe rate of 0.20 mL/min ans above, with 80:20 Water/IPA @ 1000psi
⇨ Flow Precision: 0.5% RSD
⇨ Qorking Temperature: 10 – 30 ℃
⇨ Humidity %20 – 90 RH
⇨ Degasser: Degasser module for eliminating air bubbles inside the mobile phase


⇨ Special Detector with HbA1c and Variant Analysis
⇨ Cell Length: 10mm
⇨ Working Temperature
⇨ Fixed Wavelength: 415nm